builder

Classes for generating SAM and BAM files and records for testing

This module contains utility classes for the generation of SAM and BAM files and alignment records, for use in testing.

Classes

SamBuilder

Builder for constructing one or more sam records (AlignmentSegments in pysam terms).

Provides the ability to manufacture records from minimal arguments, while generating any remaining attributes to ensure a valid record.

A builder is constructed with a handful of defaults including lengths for generated R1s and R2s, the default base quality score to use, a sequence dictionary and a single read group.

Records are then added using the add_pair() method. Once accumulated the records can be accessed in the order in which they were created through the to_unsorted_list() function, or in a list sorted by coordinate order via to_sorted_list(). The latter creates a temporary file to do the sorting and is somewhat slower as a result. Lastly, the records can be written to a temporary file using to_path().

Source code in fgpyo/sam/builder.py

class SamBuilder:
    """Builder for constructing one or more sam records (AlignmentSegments in pysam terms).

    Provides the ability to manufacture records from minimal arguments, while generating
    any remaining attributes to ensure a valid record.

    A builder is constructed with a handful of defaults including lengths for generated R1s
    and R2s, the default base quality score to use, a sequence dictionary and a single read group.

    Records are then added using the [`add_pair()`][fgpyo.sam.builder.SamBuilder.add_pair]
    method.  Once accumulated the records can be accessed in the order in which they were created
    through the [`to_unsorted_list()`][fgpyo.sam.builder.SamBuilder.to_unsorted_list]
    function, or in a list sorted by coordinate order via
    [`to_sorted_list()`][fgpyo.sam.builder.SamBuilder.to_sorted_list].  The latter creates
    a temporary file to do the sorting and is somewhat slower as a result.  Lastly, the records can
    be written to a temporary file using
    [`to_path()`][fgpyo.sam.builder.SamBuilder.to_path].
    """

    # The default read one length
    DEFAULT_R1_LENGTH: int = 100

    # The default read two length
    DEFAULT_R2_LENGTH: int = 100

    @staticmethod
    def default_sd() -> List[Dict[str, Any]]:
        """Generates the sequence dictionary that is used by default by SamBuilder.

        Matches the names and lengths of the HG19 reference in use in production.

        Returns:
            A new copy of the sequence dictionary as a list of dictionaries, one per chromosome.
        """
        return [
            {"SN": "chr1", "LN": 249250621},
            {"SN": "chr2", "LN": 243199373},
            {"SN": "chr3", "LN": 198022430},
            {"SN": "chr4", "LN": 191154276},
            {"SN": "chr5", "LN": 180915260},
            {"SN": "chr6", "LN": 171115067},
            {"SN": "chr7", "LN": 159138663},
            {"SN": "chr8", "LN": 146364022},
            {"SN": "chr9", "LN": 141213431},
            {"SN": "chr10", "LN": 135534747},
            {"SN": "chr11", "LN": 135006516},
            {"SN": "chr12", "LN": 133851895},
            {"SN": "chr13", "LN": 115169878},
            {"SN": "chr14", "LN": 107349540},
            {"SN": "chr15", "LN": 102531392},
            {"SN": "chr16", "LN": 90354753},
            {"SN": "chr17", "LN": 81195210},
            {"SN": "chr18", "LN": 78077248},
            {"SN": "chr19", "LN": 59128983},
            {"SN": "chr20", "LN": 63025520},
            {"SN": "chr21", "LN": 48129895},
            {"SN": "chr22", "LN": 51304566},
            {"SN": "chrX", "LN": 155270560},
            {"SN": "chrY", "LN": 59373566},
            {"SN": "chrM", "LN": 16571},
        ]

    @staticmethod
    def default_rg() -> Dict[str, str]:
        """Returns the default read group used by the SamBuilder, as a dictionary."""
        return {"ID": "1", "SM": "1_AAAAAA", "LB": "default", "PL": "ILLUMINA", "PU": "xxx.1"}

    def __init__(
        self,
        r1_len: Optional[int] = None,
        r2_len: Optional[int] = None,
        base_quality: int = 30,
        mapping_quality: int = 60,
        sd: Optional[List[Dict[str, Any]]] = None,
        rg: Optional[Dict[str, str]] = None,
        extra_header: Optional[Dict[str, Any]] = None,
        seed: int = 42,
        sort_order: SamOrder = SamOrder.Coordinate,
    ) -> None:
        """Initializes a new SamBuilder for generating alignment records and SAM/BAM files.

        Args:
            r1_len: The length of R1s to create unless otherwise specified
            r2_len: The length of R2s to create unless otherwise specified
            base_quality: The base quality of bases to create unless otherwise specified
            sd: a sequence dictionary as a list of dicts; defaults to calling default_sd() if None
            rg: a single read group as a dict; defaults to calling default_sd() if None
            extra_header: a dictionary of extra values to add to the header, None otherwise.  See
                          `pysam.AlignmentHeader` for more details.
            seed: a seed value for random number/string generation
            sort_order: Order to sort records when writing to file, or output of to_sorted_list()
        """

        self.r1_len: int = r1_len if r1_len is not None else self.DEFAULT_R1_LENGTH
        self.r2_len: int = r2_len if r2_len is not None else self.DEFAULT_R2_LENGTH
        self.base_quality: int = base_quality
        self.mapping_quality: int = mapping_quality

        if not isinstance(sort_order, SamOrder):
            raise ValueError(f"sort_order must be a SamOrder, got {type(sort_order)}")
        self._sort_order = sort_order

        self._header: Dict[str, Any] = {
            "HD": {"VN": "1.5", "SO": sort_order.value},
            "SQ": (sd if sd is not None else SamBuilder.default_sd()),
            "RG": [(rg if rg is not None else SamBuilder.default_rg())],
        }
        if extra_header is not None:
            self._header = {**self._header, **extra_header}
        self._samheader = AlignmentHeader.from_dict(self._header)
        self._seq_lookup = dict([(s["SN"], s) for s in self._header["SQ"]])

        self._random: Random = Random(seed)
        self._records: List[AlignedSegment] = []
        self._counter: int = 0

    def _next_name(self) -> str:
        """Returns the next available query/template name."""
        n = self._counter
        self._counter += 1
        return f"q{n:>04}"

    def _bases(self, length: int) -> str:
        """Returns a random string of bases of the length requested."""
        return "".join(self._random.choices("ACGT", k=length))

    def _new_rec(
        self,
        name: str,
        chrom: str,
        start: int,
        mapq: Optional[int],
        attrs: Optional[Dict[str, Any]],
    ) -> AlignedSegment:
        """Generates a new AlignedSegment.  Sets the segment up with the correct
        header and adds the RG attribute if not contained in attrs.

        Args:
            name: the name of the read/template
            chrom: the chromosome to which the read is mapped
            start: the start position of the read on the chromosome
            mapq: an optional mapping quality; use self.mapping_quality if None
            attrs: an optional dictionary of SAM attributes with two-char keys

        Returns:
            AlignedSegment: an aligned segment with name, chrom, pos, attributes the
                read group, and the unmapped flag all set appropriately.
        """
        if chrom is not sam.NO_REF_NAME and chrom not in self._seq_lookup:
            raise ValueError(f"{chrom} is not a valid chromosome name in this builder.")

        rec = AlignedSegment(header=self._samheader)
        rec.query_name = name
        rec.reference_name = chrom
        rec.reference_start = start
        rec.mapping_quality = mapq if mapq is not None else self.mapping_quality

        if chrom == sam.NO_REF_NAME or start == sam.NO_REF_POS:
            rec.is_unmapped = True
            rec.mapping_quality = 0

        attrs = attrs if attrs else dict()
        if "RG" not in attrs:
            attrs["RG"] = self.rg_id()
        rec.set_tags(list(attrs.items()))
        return rec

    def _set_flags(
        self,
        rec: pysam.AlignedSegment,
        read_num: Optional[int],
        strand: str,
        secondary: bool = False,
        supplementary: bool = False,
    ) -> None:
        """Appropriately sets most flag fields on the given read.

        Args:
            rec: the read to set the flags on
            read_num: Either None for an unpaired read, or 1 or 2
            strand: Either "+" or "-" to indicate strand of the read
        """
        rec.is_paired = read_num is not None
        rec.is_read1 = read_num == 1
        rec.is_read2 = read_num == 2
        rec.is_qcfail = False
        rec.is_duplicate = False
        rec.is_secondary = secondary
        rec.is_supplementary = supplementary
        if not rec.is_unmapped:
            rec.is_reverse = strand != "+"

    def _set_length_dependent_fields(
        self,
        rec: pysam.AlignedSegment,
        length: int,
        bases: Optional[str] = None,
        quals: Optional[List[int]] = None,
        cigar: Optional[str] = None,
    ) -> None:
        """Fills in bases, quals and cigar on a record.

        If any of bases, quals or cigar are defined, they must all have the same length/query
        length.  If none are defined then the length parameter is used.  Undefined values are
        synthesize at the inferred length.

        Args:
            rec: a SAM record
            length: the length to use if all of bases/quals/cigar are None
            bases: an optional string of bases for the read
            quals: an optional list of qualities for the read
            cigar: an optional cigar string for the read
        """

        # Do some validation to make sure all defined things have the same lengths
        lengths = set()
        if bases is not None:
            lengths.add(len(bases))
        if quals is not None:
            lengths.add(len(quals))
        if cigar is not None:
            cig = sam.Cigar.from_cigarstring(cigar)
            lengths.add(sum([elem.length_on_query for elem in cig.elements]))

        if not lengths:
            lengths.add(length)

        if len(lengths) != 1:
            raise ValueError("Provided bases/quals/cigar are not length compatible.")

        # Fill in the record, making any parts that were not defined as params
        length = lengths.pop()
        query_quals = array("B", quals if quals else [self.base_quality] * length)
        rec.query_sequence = bases if bases else self._bases(length)
        rec.query_qualities = query_quals
        if not rec.is_unmapped:
            rec.cigarstring = cigar if cigar else f"{length}M"

    def rg(self) -> Dict[str, Any]:
        """Returns the single read group that is defined in the header."""
        # The `RG` field contains a list of read group mappings
        # e.g. `[{"ID": "rg1", "PL": "ILLUMINA"}]`
        rgs = cast(List[Dict[str, Any]], self._header["RG"])
        assert len(rgs) == 1, "Header did not contain exactly one read group!"
        return rgs[0]

    def rg_id(self) -> str:
        """Returns the ID of the single read group that is defined in the header."""
        # The read group mapping has mixed types of values (e.g. "PI" is numeric), but the "ID"
        # field is always a string.
        return cast(str, self.rg()["ID"])

    def add_pair(
        self,
        *,
        name: Optional[str] = None,
        bases1: Optional[str] = None,
        bases2: Optional[str] = None,
        quals1: Optional[List[int]] = None,
        quals2: Optional[List[int]] = None,
        chrom: Optional[str] = None,
        chrom1: Optional[str] = None,
        chrom2: Optional[str] = None,
        start1: int = sam.NO_REF_POS,
        start2: int = sam.NO_REF_POS,
        cigar1: Optional[str] = None,
        cigar2: Optional[str] = None,
        mapq1: Optional[int] = None,
        mapq2: Optional[int] = None,
        strand1: str = "+",
        strand2: str = "-",
        attrs: Optional[Dict[str, Any]] = None,
    ) -> Tuple[AlignedSegment, AlignedSegment]:
        """Generates a new pair of reads, adds them to the internal collection, and returns them.

        Most fields are optional.

        Mapped pairs can be created by specifying both `start1` and `start2` and either `chrom`, for
        pairs where both reads map to the same contig, or both `chrom1` and `chrom2`, for pairs
        where reads map to different contigs. i.e.:

            - `add_pair(chrom, start1, start2)` will create a mapped pair where both reads map to
              the same contig (`chrom`).
            - `add_pair(chrom1, start1, chrom2, start2)` will create a mapped pair where the reads
              map to different contigs (`chrom1` and `chrom2`).

        A pair with only one of the two reads mapped can be created by setting only one start
        position. Flags will automatically be set correctly for the unmapped mate.

            - `add_pair(chrom, start1)`
            - `add_pair(chrom1, start1)`
            - `add_pair(chrom, start2)`
            - `add_pair(chrom2, start2)`

        An unmapped pair can be created by calling the method with no parameters (specifically,
        not setting `chrom`, `chrom1`, `start1`, `chrom2`, or `start2`). If either cigar is
        provided, it will be ignored.

        For a given read (i.e. R1 or R2) the length of the read is determined based on the presence
        or absence of bases, quals, and cigar.  If values are provided for one or more of these
        parameters, the lengths must match, and the length will be used to generate any
        unsupplied values.  If none of bases, quals, and cigar are provided, all three will be
        synthesized based on either the r1_len or r2_len stored on the class as appropriate.

        When synthesizing, bases are always a random sequence of bases, quals are all the default
        base quality (supplied when constructing a SamBuilder) and the cigar is always a single M
        operator of the read length.

        Args:
            name: The name of the template. If None is given a unique name will be auto-generated.
            bases1: The bases for R1. If None is given a random sequence is generated.
            bases2: The bases for R2. If None is given a random sequence is generated.
            quals1: The list of int qualities for R1. If None, the default base quality is used.
            quals2: The list of int qualities for R2. If None, the default base quality is used.
            chrom: The chromosome to which both reads are mapped. Defaults to the unmapped value.
            chrom1: The chromosome to which R1 is mapped. If None, `chrom` is used.
            chrom2: The chromosome to which R2 is mapped. If None, `chrom` is used.
            start1: The start position of R1. Defaults to the unmapped value.
            start2: The start position of R2. Defaults to the unmapped value.
            cigar1: The cigar string for R1. Defaults to None for unmapped reads, otherwise all M.
            cigar2: The cigar string for R2. Defaults to None for unmapped reads, otherwise all M.
            mapq1: Mapping quality for R1. Defaults to self.mapping_quality if None.
            mapq2: Mapping quality for R2. Defaults to self.mapping_quality if None.
            strand1: The strand for R1, either "+" or "-". Defaults to "+".
            strand2: The strand for R2, either "+" or "-". Defaults to "-".
            attrs: An optional dictionary of SAM attribute to place on both R1 and R2.

        Raises:
            ValueError: if either strand field is not "+" or "-"
            ValueError: if bases/quals/cigar are set in a way that is not self-consistent

        Returns:
            Tuple[AlignedSegment, AlignedSegment]: The pair of records created, R1 then R2.
        """

        if strand1 not in ["+", "-"]:
            raise ValueError(f"Invalid value for strand1: {strand1}")
        if strand2 not in ["+", "-"]:
            raise ValueError(f"Invalid value for strand2: {strand2}")

        name = name if name is not None else self._next_name()

        # Valid parameterizations for contig mapping (backward compatible):
        # - chrom, start1, start2
        # - chrom, start1
        # - chrom, start2
        # Valid parameterizations for contig mapping (new):
        # - chrom1, start1, chrom2, start2
        # - chrom1, start1
        # - chrom2, start2
        if chrom is not None and (chrom1 is not None or chrom2 is not None):
            raise ValueError("Cannot use chrom in combination with chrom1 or chrom2")

        chrom = sam.NO_REF_NAME if chrom is None else chrom

        if start1 != sam.NO_REF_POS:
            chrom1 = next(c for c in (chrom1, chrom) if c is not None)
        else:
            chrom1 = sam.NO_REF_NAME

        if start2 != sam.NO_REF_POS:
            chrom2 = next(c for c in (chrom2, chrom) if c is not None)
        else:
            chrom2 = sam.NO_REF_NAME

        if chrom1 == sam.NO_REF_NAME and start1 != sam.NO_REF_POS:
            raise ValueError("start1 cannot be used on its own - specify chrom or chrom1")

        if chrom2 == sam.NO_REF_NAME and start2 != sam.NO_REF_POS:
            raise ValueError("start2 cannot be used on its own - specify chrom or chrom2")

        # Setup R1
        r1 = self._new_rec(name=name, chrom=chrom1, start=start1, mapq=mapq1, attrs=attrs)
        self._set_flags(r1, read_num=1, strand=strand1)
        self._set_length_dependent_fields(
            rec=r1, length=self.r1_len, bases=bases1, quals=quals1, cigar=cigar1
        )

        # Setup R2
        r2 = self._new_rec(name=name, chrom=chrom2, start=start2, mapq=mapq2, attrs=attrs)
        self._set_flags(r2, read_num=2, strand=strand2)
        self._set_length_dependent_fields(
            rec=r2, length=self.r2_len, bases=bases2, quals=quals2, cigar=cigar2
        )

        # Sync up mate info and we're done!
        sam.set_mate_info(r1, r2)
        self._records.append(r1)
        self._records.append(r2)
        return r1, r2

    def add_single(
        self,
        *,
        name: Optional[str] = None,
        read_num: Optional[int] = None,
        bases: Optional[str] = None,
        quals: Optional[List[int]] = None,
        chrom: str = sam.NO_REF_NAME,
        start: int = sam.NO_REF_POS,
        cigar: Optional[str] = None,
        mapq: Optional[int] = None,
        strand: str = "+",
        secondary: bool = False,
        supplementary: bool = False,
        attrs: Optional[Dict[str, Any]] = None,
    ) -> AlignedSegment:
        """Generates a new single reads, adds them to the internal collection, and returns it.

        Most fields are optional.

        If `read_num` is None (the default) an unpaired read will be created.  If `read_num` is
        set to 1 or 2, the read will have it's paired flag set and read number flags set.

        An unmapped read can be created by calling the method with no parameters (specifically,
        not setting chrom, start1 or start2).  If cigar is provided, it will be ignored.

        A mapped read is created by providing chrom and start.

        The length of the read is determined based on the presence or absence of bases, quals,
        and cigar.  If values are provided for one or more of these parameters, the lengths must
        match, and the length will be used to generate any unsupplied values.  If none of bases,
        quals, and cigar are provided, all three will be synthesized based on either the r1_len
        or r2_len stored on the class as appropriate.

        When synthesizing, bases are always a random sequence of bases, quals are all the default
        base quality (supplied when constructing a SamBuilder) and the cigar is always a single M
        operator of the read length.

        Args:
            name: The name of the template. If None is given a unique name will be auto-generated.
            read_num: Either None, 1 for R1 or 2 for R2
            bases: The bases for the read. If None is given a random sequence is generated.
            quals: The list of qualities for the read. If None, the default base quality is used.
            chrom: The chromosome to which both reads are mapped. Defaults to the unmapped value.
            start: The start position of the read. Defaults to the unmapped value.
            cigar: The cigar string for R1. Defaults to None for unmapped reads, otherwise all M.
            mapq: Mapping quality for the read. Default to self.mapping_quality if not given.
            strand: The strand for R1, either "+" or "-". Defaults to "+".
            secondary: If true the read will be flagged as secondary
            supplementary: If true the read will be flagged as supplementary
            attrs: An optional dictionary of SAM attribute to place on both R1 and R2.

        Raises:
            ValueError: if strand field is not "+" or "-"
            ValueError: if read_num is not None, 1 or 2
            ValueError: if bases/quals/cigar are set in a way that is not self-consistent

        Returns:
            AlignedSegment: The record created
        """

        if strand not in ["+", "-"]:
            raise ValueError(f"Invalid value for strand1: {strand}")
        if read_num not in [None, 1, 2]:
            raise ValueError(f"Invalid value for read_num: {read_num}")

        name = name if name is not None else self._next_name()

        # Setup the read
        read_len = self.r1_len if read_num != 2 else self.r2_len
        rec = self._new_rec(name=name, chrom=chrom, start=start, mapq=mapq, attrs=attrs)
        self._set_flags(
            rec, read_num=read_num, strand=strand, secondary=secondary, supplementary=supplementary
        )
        self._set_length_dependent_fields(
            rec=rec, length=read_len, bases=bases, quals=quals, cigar=cigar
        )

        self._records.append(rec)
        return rec

    def to_path(  # noqa: C901
        self,
        path: Optional[Path] = None,
        index: bool = True,
        pred: Callable[[AlignedSegment], bool] = lambda r: True,
        tmp_file_type: Optional[sam.SamFileType] = None,
    ) -> Path:
        """Write the accumulated records to a file, sorts & indexes it, and returns the Path.
        If a path is provided, it will be written to, otherwise a temporary file is created
        and returned.

        If `path` is provided, `tmp_file_type` may not be provided. In this case, the file type
        (SAM/BAM/CRAM) will be automatically determined by the file extension when a path
        is provided.  See `~pysam` for more details.

        If `path` is not provided, the file type will default to BAM unless `tmp_file_type` is
        provided.

        Args:
            path: a path at which to write the file, otherwise a temp file is used.
            index: if True and `sort_order` is `Coordinate` and output is a BAM/CRAM file, then
                   an index is generated, otherwise not.
            pred: optional predicate to specify which reads should be output
            tmp_file_type: the file type to output when a path is not provided (default is BAM)

        Returns:
            Path: The path to the sorted (and possibly indexed) file.
        """
        if path is not None:
            # Get the file type if a path was given (in this case, a file type may not be
            # provided too)
            if tmp_file_type is not None:
                raise ValueError("Both `path` and `tmp_file_type` cannot be provided.")
            tmp_file_type = sam.SamFileType.from_path(path)
        elif tmp_file_type is None:
            # Use the provided file type
            tmp_file_type = sam.SamFileType.BAM

        # Get the extension, and create a path if none was given
        ext = tmp_file_type.extension
        if path is None:
            with NamedTemporaryFile(suffix=ext, delete=False) as fp:
                path = Path(fp.name)

        with NamedTemporaryFile(suffix=ext, delete=True) as fp:
            file_handle: IO
            if self._sort_order in {SamOrder.Unsorted, SamOrder.Unknown}:
                file_handle = path.open("w")
            else:
                file_handle = fp.file

            with sam.writer(file_handle, header=self._samheader, file_type=tmp_file_type) as writer:
                for rec in self._records:
                    if pred(rec):
                        writer.write(rec)

            samtools_sort_args = ["-o", str(path), fp.name]

            file_handle.close()
            if self._sort_order == SamOrder.QueryName:
                pysam.sort("-n", *samtools_sort_args)
            elif self._sort_order == SamOrder.Coordinate:
                if index and tmp_file_type.indexable:
                    samtools_sort_args.insert(0, "--write-index")
                pysam.sort(*samtools_sort_args)

        return path

    def __len__(self) -> int:
        """Returns the number of records accumulated so far."""
        return len(self._records)

    def to_unsorted_list(self) -> List[pysam.AlignedSegment]:
        """Returns the accumulated records in the order they were created."""
        return list(self._records)

    def to_sorted_list(self) -> List[pysam.AlignedSegment]:
        """Returns the accumulated records in coordinate order."""
        with NamedTemporaryFile(suffix=".bam", delete=True) as fp:
            filename = fp.name
            path = self.to_path(path=Path(filename), index=False)
            bam = sam.reader(path)
            return list(bam)

    @property
    def header(self) -> AlignmentHeader:
        """Returns the builder's SAM header."""
        return self._samheader

Attributes

header property

header: AlignmentHeader

Returns the builder's SAM header.

Functions

__init__

__init__(r1_len: Optional[int] = None, r2_len: Optional[int] = None, base_quality: int = 30, mapping_quality: int = 60, sd: Optional[List[Dict[str, Any]]] = None, rg: Optional[Dict[str, str]] = None, extra_header: Optional[Dict[str, Any]] = None, seed: int = 42, sort_order: SamOrder = Coordinate) -> None

Initializes a new SamBuilder for generating alignment records and SAM/BAM files.

Parameters:

Name	Type	Description	Default
r1_len	Optional[int]	The length of R1s to create unless otherwise specified	None
r2_len	Optional[int]	The length of R2s to create unless otherwise specified	None
base_quality	int	The base quality of bases to create unless otherwise specified	30
sd	Optional[List[Dict[str, Any]]]	a sequence dictionary as a list of dicts; defaults to calling default_sd() if None	None
rg	Optional[Dict[str, str]]	a single read group as a dict; defaults to calling default_sd() if None	None
extra_header	Optional[Dict[str, Any]]	a dictionary of extra values to add to the header, None otherwise. See pysam.AlignmentHeader for more details.	None
seed	int	a seed value for random number/string generation	42
sort_order	SamOrder	Order to sort records when writing to file, or output of to_sorted_list()	Coordinate

Source code in fgpyo/sam/builder.py

def __init__(
    self,
    r1_len: Optional[int] = None,
    r2_len: Optional[int] = None,
    base_quality: int = 30,
    mapping_quality: int = 60,
    sd: Optional[List[Dict[str, Any]]] = None,
    rg: Optional[Dict[str, str]] = None,
    extra_header: Optional[Dict[str, Any]] = None,
    seed: int = 42,
    sort_order: SamOrder = SamOrder.Coordinate,
) -> None:
    """Initializes a new SamBuilder for generating alignment records and SAM/BAM files.

    Args:
        r1_len: The length of R1s to create unless otherwise specified
        r2_len: The length of R2s to create unless otherwise specified
        base_quality: The base quality of bases to create unless otherwise specified
        sd: a sequence dictionary as a list of dicts; defaults to calling default_sd() if None
        rg: a single read group as a dict; defaults to calling default_sd() if None
        extra_header: a dictionary of extra values to add to the header, None otherwise.  See
                      `pysam.AlignmentHeader` for more details.
        seed: a seed value for random number/string generation
        sort_order: Order to sort records when writing to file, or output of to_sorted_list()
    """

    self.r1_len: int = r1_len if r1_len is not None else self.DEFAULT_R1_LENGTH
    self.r2_len: int = r2_len if r2_len is not None else self.DEFAULT_R2_LENGTH
    self.base_quality: int = base_quality
    self.mapping_quality: int = mapping_quality

    if not isinstance(sort_order, SamOrder):
        raise ValueError(f"sort_order must be a SamOrder, got {type(sort_order)}")
    self._sort_order = sort_order

    self._header: Dict[str, Any] = {
        "HD": {"VN": "1.5", "SO": sort_order.value},
        "SQ": (sd if sd is not None else SamBuilder.default_sd()),
        "RG": [(rg if rg is not None else SamBuilder.default_rg())],
    }
    if extra_header is not None:
        self._header = {**self._header, **extra_header}
    self._samheader = AlignmentHeader.from_dict(self._header)
    self._seq_lookup = dict([(s["SN"], s) for s in self._header["SQ"]])

    self._random: Random = Random(seed)
    self._records: List[AlignedSegment] = []
    self._counter: int = 0

__len__

__len__() -> int

Returns the number of records accumulated so far.

Source code in fgpyo/sam/builder.py

def __len__(self) -> int:
    """Returns the number of records accumulated so far."""
    return len(self._records)

add_pair

add_pair(*, name: Optional[str] = None, bases1: Optional[str] = None, bases2: Optional[str] = None, quals1: Optional[List[int]] = None, quals2: Optional[List[int]] = None, chrom: Optional[str] = None, chrom1: Optional[str] = None, chrom2: Optional[str] = None, start1: int = NO_REF_POS, start2: int = NO_REF_POS, cigar1: Optional[str] = None, cigar2: Optional[str] = None, mapq1: Optional[int] = None, mapq2: Optional[int] = None, strand1: str = '+', strand2: str = '-', attrs: Optional[Dict[str, Any]] = None) -> Tuple[AlignedSegment, AlignedSegment]

Generates a new pair of reads, adds them to the internal collection, and returns them.

Most fields are optional.

Mapped pairs can be created by specifying both start1 and start2 and either chrom, for pairs where both reads map to the same contig, or both chrom1 and chrom2, for pairs where reads map to different contigs. i.e.:

- `add_pair(chrom, start1, start2)` will create a mapped pair where both reads map to
  the same contig (`chrom`).
- `add_pair(chrom1, start1, chrom2, start2)` will create a mapped pair where the reads
  map to different contigs (`chrom1` and `chrom2`).

A pair with only one of the two reads mapped can be created by setting only one start position. Flags will automatically be set correctly for the unmapped mate.

- `add_pair(chrom, start1)`
- `add_pair(chrom1, start1)`
- `add_pair(chrom, start2)`
- `add_pair(chrom2, start2)`

An unmapped pair can be created by calling the method with no parameters (specifically, not setting chrom, chrom1, start1, chrom2, or start2). If either cigar is provided, it will be ignored.

For a given read (i.e. R1 or R2) the length of the read is determined based on the presence or absence of bases, quals, and cigar. If values are provided for one or more of these parameters, the lengths must match, and the length will be used to generate any unsupplied values. If none of bases, quals, and cigar are provided, all three will be synthesized based on either the r1_len or r2_len stored on the class as appropriate.

When synthesizing, bases are always a random sequence of bases, quals are all the default base quality (supplied when constructing a SamBuilder) and the cigar is always a single M operator of the read length.

Parameters:

Name	Type	Description	Default
name	Optional[str]	The name of the template. If None is given a unique name will be auto-generated.	None
bases1	Optional[str]	The bases for R1. If None is given a random sequence is generated.	None
bases2	Optional[str]	The bases for R2. If None is given a random sequence is generated.	None
quals1	Optional[List[int]]	The list of int qualities for R1. If None, the default base quality is used.	None
quals2	Optional[List[int]]	The list of int qualities for R2. If None, the default base quality is used.	None
chrom	Optional[str]	The chromosome to which both reads are mapped. Defaults to the unmapped value.	None
chrom1	Optional[str]	The chromosome to which R1 is mapped. If None, chrom is used.	None
chrom2	Optional[str]	The chromosome to which R2 is mapped. If None, chrom is used.	None
start1	int	The start position of R1. Defaults to the unmapped value.	NO_REF_POS
start2	int	The start position of R2. Defaults to the unmapped value.	NO_REF_POS
cigar1	Optional[str]	The cigar string for R1. Defaults to None for unmapped reads, otherwise all M.	None
cigar2	Optional[str]	The cigar string for R2. Defaults to None for unmapped reads, otherwise all M.	None
mapq1	Optional[int]	Mapping quality for R1. Defaults to self.mapping_quality if None.	None
mapq2	Optional[int]	Mapping quality for R2. Defaults to self.mapping_quality if None.	None
strand1	str	The strand for R1, either "+" or "-". Defaults to "+".	'+'
strand2	str	The strand for R2, either "+" or "-". Defaults to "-".	'-'
attrs	Optional[Dict[str, Any]]	An optional dictionary of SAM attribute to place on both R1 and R2.	None

Raises:

Type	Description
ValueError	if either strand field is not "+" or "-"
ValueError	if bases/quals/cigar are set in a way that is not self-consistent

Returns:

Type	Description
Tuple[AlignedSegment, AlignedSegment]	Tuple[AlignedSegment, AlignedSegment]: The pair of records created, R1 then R2.

Source code in fgpyo/sam/builder.py

def add_pair(
    self,
    *,
    name: Optional[str] = None,
    bases1: Optional[str] = None,
    bases2: Optional[str] = None,
    quals1: Optional[List[int]] = None,
    quals2: Optional[List[int]] = None,
    chrom: Optional[str] = None,
    chrom1: Optional[str] = None,
    chrom2: Optional[str] = None,
    start1: int = sam.NO_REF_POS,
    start2: int = sam.NO_REF_POS,
    cigar1: Optional[str] = None,
    cigar2: Optional[str] = None,
    mapq1: Optional[int] = None,
    mapq2: Optional[int] = None,
    strand1: str = "+",
    strand2: str = "-",
    attrs: Optional[Dict[str, Any]] = None,
) -> Tuple[AlignedSegment, AlignedSegment]:
    """Generates a new pair of reads, adds them to the internal collection, and returns them.

    Most fields are optional.

    Mapped pairs can be created by specifying both `start1` and `start2` and either `chrom`, for
    pairs where both reads map to the same contig, or both `chrom1` and `chrom2`, for pairs
    where reads map to different contigs. i.e.:

        - `add_pair(chrom, start1, start2)` will create a mapped pair where both reads map to
          the same contig (`chrom`).
        - `add_pair(chrom1, start1, chrom2, start2)` will create a mapped pair where the reads
          map to different contigs (`chrom1` and `chrom2`).

    A pair with only one of the two reads mapped can be created by setting only one start
    position. Flags will automatically be set correctly for the unmapped mate.

        - `add_pair(chrom, start1)`
        - `add_pair(chrom1, start1)`
        - `add_pair(chrom, start2)`
        - `add_pair(chrom2, start2)`

    An unmapped pair can be created by calling the method with no parameters (specifically,
    not setting `chrom`, `chrom1`, `start1`, `chrom2`, or `start2`). If either cigar is
    provided, it will be ignored.

    For a given read (i.e. R1 or R2) the length of the read is determined based on the presence
    or absence of bases, quals, and cigar.  If values are provided for one or more of these
    parameters, the lengths must match, and the length will be used to generate any
    unsupplied values.  If none of bases, quals, and cigar are provided, all three will be
    synthesized based on either the r1_len or r2_len stored on the class as appropriate.

    When synthesizing, bases are always a random sequence of bases, quals are all the default
    base quality (supplied when constructing a SamBuilder) and the cigar is always a single M
    operator of the read length.

    Args:
        name: The name of the template. If None is given a unique name will be auto-generated.
        bases1: The bases for R1. If None is given a random sequence is generated.
        bases2: The bases for R2. If None is given a random sequence is generated.
        quals1: The list of int qualities for R1. If None, the default base quality is used.
        quals2: The list of int qualities for R2. If None, the default base quality is used.
        chrom: The chromosome to which both reads are mapped. Defaults to the unmapped value.
        chrom1: The chromosome to which R1 is mapped. If None, `chrom` is used.
        chrom2: The chromosome to which R2 is mapped. If None, `chrom` is used.
        start1: The start position of R1. Defaults to the unmapped value.
        start2: The start position of R2. Defaults to the unmapped value.
        cigar1: The cigar string for R1. Defaults to None for unmapped reads, otherwise all M.
        cigar2: The cigar string for R2. Defaults to None for unmapped reads, otherwise all M.
        mapq1: Mapping quality for R1. Defaults to self.mapping_quality if None.
        mapq2: Mapping quality for R2. Defaults to self.mapping_quality if None.
        strand1: The strand for R1, either "+" or "-". Defaults to "+".
        strand2: The strand for R2, either "+" or "-". Defaults to "-".
        attrs: An optional dictionary of SAM attribute to place on both R1 and R2.

    Raises:
        ValueError: if either strand field is not "+" or "-"
        ValueError: if bases/quals/cigar are set in a way that is not self-consistent

    Returns:
        Tuple[AlignedSegment, AlignedSegment]: The pair of records created, R1 then R2.
    """

    if strand1 not in ["+", "-"]:
        raise ValueError(f"Invalid value for strand1: {strand1}")
    if strand2 not in ["+", "-"]:
        raise ValueError(f"Invalid value for strand2: {strand2}")

    name = name if name is not None else self._next_name()

    # Valid parameterizations for contig mapping (backward compatible):
    # - chrom, start1, start2
    # - chrom, start1
    # - chrom, start2
    # Valid parameterizations for contig mapping (new):
    # - chrom1, start1, chrom2, start2
    # - chrom1, start1
    # - chrom2, start2
    if chrom is not None and (chrom1 is not None or chrom2 is not None):
        raise ValueError("Cannot use chrom in combination with chrom1 or chrom2")

    chrom = sam.NO_REF_NAME if chrom is None else chrom

    if start1 != sam.NO_REF_POS:
        chrom1 = next(c for c in (chrom1, chrom) if c is not None)
    else:
        chrom1 = sam.NO_REF_NAME

    if start2 != sam.NO_REF_POS:
        chrom2 = next(c for c in (chrom2, chrom) if c is not None)
    else:
        chrom2 = sam.NO_REF_NAME

    if chrom1 == sam.NO_REF_NAME and start1 != sam.NO_REF_POS:
        raise ValueError("start1 cannot be used on its own - specify chrom or chrom1")

    if chrom2 == sam.NO_REF_NAME and start2 != sam.NO_REF_POS:
        raise ValueError("start2 cannot be used on its own - specify chrom or chrom2")

    # Setup R1
    r1 = self._new_rec(name=name, chrom=chrom1, start=start1, mapq=mapq1, attrs=attrs)
    self._set_flags(r1, read_num=1, strand=strand1)
    self._set_length_dependent_fields(
        rec=r1, length=self.r1_len, bases=bases1, quals=quals1, cigar=cigar1
    )

    # Setup R2
    r2 = self._new_rec(name=name, chrom=chrom2, start=start2, mapq=mapq2, attrs=attrs)
    self._set_flags(r2, read_num=2, strand=strand2)
    self._set_length_dependent_fields(
        rec=r2, length=self.r2_len, bases=bases2, quals=quals2, cigar=cigar2
    )

    # Sync up mate info and we're done!
    sam.set_mate_info(r1, r2)
    self._records.append(r1)
    self._records.append(r2)
    return r1, r2

add_single

add_single(*, name: Optional[str] = None, read_num: Optional[int] = None, bases: Optional[str] = None, quals: Optional[List[int]] = None, chrom: str = NO_REF_NAME, start: int = NO_REF_POS, cigar: Optional[str] = None, mapq: Optional[int] = None, strand: str = '+', secondary: bool = False, supplementary: bool = False, attrs: Optional[Dict[str, Any]] = None) -> AlignedSegment

Generates a new single reads, adds them to the internal collection, and returns it.

Most fields are optional.

If read_num is None (the default) an unpaired read will be created. If read_num is set to 1 or 2, the read will have it's paired flag set and read number flags set.

An unmapped read can be created by calling the method with no parameters (specifically, not setting chrom, start1 or start2). If cigar is provided, it will be ignored.

A mapped read is created by providing chrom and start.

The length of the read is determined based on the presence or absence of bases, quals, and cigar. If values are provided for one or more of these parameters, the lengths must match, and the length will be used to generate any unsupplied values. If none of bases, quals, and cigar are provided, all three will be synthesized based on either the r1_len or r2_len stored on the class as appropriate.

When synthesizing, bases are always a random sequence of bases, quals are all the default base quality (supplied when constructing a SamBuilder) and the cigar is always a single M operator of the read length.

Parameters:

Name	Type	Description	Default
name	Optional[str]	The name of the template. If None is given a unique name will be auto-generated.	None
read_num	Optional[int]	Either None, 1 for R1 or 2 for R2	None
bases	Optional[str]	The bases for the read. If None is given a random sequence is generated.	None
quals	Optional[List[int]]	The list of qualities for the read. If None, the default base quality is used.	None
chrom	str	The chromosome to which both reads are mapped. Defaults to the unmapped value.	NO_REF_NAME
start	int	The start position of the read. Defaults to the unmapped value.	NO_REF_POS
cigar	Optional[str]	The cigar string for R1. Defaults to None for unmapped reads, otherwise all M.	None
mapq	Optional[int]	Mapping quality for the read. Default to self.mapping_quality if not given.	None
strand	str	The strand for R1, either "+" or "-". Defaults to "+".	'+'
secondary	bool	If true the read will be flagged as secondary	False
supplementary	bool	If true the read will be flagged as supplementary	False
attrs	Optional[Dict[str, Any]]	An optional dictionary of SAM attribute to place on both R1 and R2.	None

Raises:

Type	Description
ValueError	if strand field is not "+" or "-"
ValueError	if read_num is not None, 1 or 2
ValueError	if bases/quals/cigar are set in a way that is not self-consistent

Returns:

Name	Type	Description
AlignedSegment	AlignedSegment	The record created

Source code in fgpyo/sam/builder.py

def add_single(
    self,
    *,
    name: Optional[str] = None,
    read_num: Optional[int] = None,
    bases: Optional[str] = None,
    quals: Optional[List[int]] = None,
    chrom: str = sam.NO_REF_NAME,
    start: int = sam.NO_REF_POS,
    cigar: Optional[str] = None,
    mapq: Optional[int] = None,
    strand: str = "+",
    secondary: bool = False,
    supplementary: bool = False,
    attrs: Optional[Dict[str, Any]] = None,
) -> AlignedSegment:
    """Generates a new single reads, adds them to the internal collection, and returns it.

    Most fields are optional.

    If `read_num` is None (the default) an unpaired read will be created.  If `read_num` is
    set to 1 or 2, the read will have it's paired flag set and read number flags set.

    An unmapped read can be created by calling the method with no parameters (specifically,
    not setting chrom, start1 or start2).  If cigar is provided, it will be ignored.

    A mapped read is created by providing chrom and start.

    The length of the read is determined based on the presence or absence of bases, quals,
    and cigar.  If values are provided for one or more of these parameters, the lengths must
    match, and the length will be used to generate any unsupplied values.  If none of bases,
    quals, and cigar are provided, all three will be synthesized based on either the r1_len
    or r2_len stored on the class as appropriate.

    When synthesizing, bases are always a random sequence of bases, quals are all the default
    base quality (supplied when constructing a SamBuilder) and the cigar is always a single M
    operator of the read length.

    Args:
        name: The name of the template. If None is given a unique name will be auto-generated.
        read_num: Either None, 1 for R1 or 2 for R2
        bases: The bases for the read. If None is given a random sequence is generated.
        quals: The list of qualities for the read. If None, the default base quality is used.
        chrom: The chromosome to which both reads are mapped. Defaults to the unmapped value.
        start: The start position of the read. Defaults to the unmapped value.
        cigar: The cigar string for R1. Defaults to None for unmapped reads, otherwise all M.
        mapq: Mapping quality for the read. Default to self.mapping_quality if not given.
        strand: The strand for R1, either "+" or "-". Defaults to "+".
        secondary: If true the read will be flagged as secondary
        supplementary: If true the read will be flagged as supplementary
        attrs: An optional dictionary of SAM attribute to place on both R1 and R2.

    Raises:
        ValueError: if strand field is not "+" or "-"
        ValueError: if read_num is not None, 1 or 2
        ValueError: if bases/quals/cigar are set in a way that is not self-consistent

    Returns:
        AlignedSegment: The record created
    """

    if strand not in ["+", "-"]:
        raise ValueError(f"Invalid value for strand1: {strand}")
    if read_num not in [None, 1, 2]:
        raise ValueError(f"Invalid value for read_num: {read_num}")

    name = name if name is not None else self._next_name()

    # Setup the read
    read_len = self.r1_len if read_num != 2 else self.r2_len
    rec = self._new_rec(name=name, chrom=chrom, start=start, mapq=mapq, attrs=attrs)
    self._set_flags(
        rec, read_num=read_num, strand=strand, secondary=secondary, supplementary=supplementary
    )
    self._set_length_dependent_fields(
        rec=rec, length=read_len, bases=bases, quals=quals, cigar=cigar
    )

    self._records.append(rec)
    return rec

default_rg staticmethod

default_rg() -> Dict[str, str]

Returns the default read group used by the SamBuilder, as a dictionary.

Source code in fgpyo/sam/builder.py

@staticmethod
def default_rg() -> Dict[str, str]:
    """Returns the default read group used by the SamBuilder, as a dictionary."""
    return {"ID": "1", "SM": "1_AAAAAA", "LB": "default", "PL": "ILLUMINA", "PU": "xxx.1"}

default_sd staticmethod

default_sd() -> List[Dict[str, Any]]

Generates the sequence dictionary that is used by default by SamBuilder.

Matches the names and lengths of the HG19 reference in use in production.

Returns:

Type	Description
List[Dict[str, Any]]	A new copy of the sequence dictionary as a list of dictionaries, one per chromosome.

Source code in fgpyo/sam/builder.py

@staticmethod
def default_sd() -> List[Dict[str, Any]]:
    """Generates the sequence dictionary that is used by default by SamBuilder.

    Matches the names and lengths of the HG19 reference in use in production.

    Returns:
        A new copy of the sequence dictionary as a list of dictionaries, one per chromosome.
    """
    return [
        {"SN": "chr1", "LN": 249250621},
        {"SN": "chr2", "LN": 243199373},
        {"SN": "chr3", "LN": 198022430},
        {"SN": "chr4", "LN": 191154276},
        {"SN": "chr5", "LN": 180915260},
        {"SN": "chr6", "LN": 171115067},
        {"SN": "chr7", "LN": 159138663},
        {"SN": "chr8", "LN": 146364022},
        {"SN": "chr9", "LN": 141213431},
        {"SN": "chr10", "LN": 135534747},
        {"SN": "chr11", "LN": 135006516},
        {"SN": "chr12", "LN": 133851895},
        {"SN": "chr13", "LN": 115169878},
        {"SN": "chr14", "LN": 107349540},
        {"SN": "chr15", "LN": 102531392},
        {"SN": "chr16", "LN": 90354753},
        {"SN": "chr17", "LN": 81195210},
        {"SN": "chr18", "LN": 78077248},
        {"SN": "chr19", "LN": 59128983},
        {"SN": "chr20", "LN": 63025520},
        {"SN": "chr21", "LN": 48129895},
        {"SN": "chr22", "LN": 51304566},
        {"SN": "chrX", "LN": 155270560},
        {"SN": "chrY", "LN": 59373566},
        {"SN": "chrM", "LN": 16571},
    ]

rg

rg() -> Dict[str, Any]

Returns the single read group that is defined in the header.

Source code in fgpyo/sam/builder.py

def rg(self) -> Dict[str, Any]:
    """Returns the single read group that is defined in the header."""
    # The `RG` field contains a list of read group mappings
    # e.g. `[{"ID": "rg1", "PL": "ILLUMINA"}]`
    rgs = cast(List[Dict[str, Any]], self._header["RG"])
    assert len(rgs) == 1, "Header did not contain exactly one read group!"
    return rgs[0]

rg_id

rg_id() -> str

Returns the ID of the single read group that is defined in the header.

Source code in fgpyo/sam/builder.py

def rg_id(self) -> str:
    """Returns the ID of the single read group that is defined in the header."""
    # The read group mapping has mixed types of values (e.g. "PI" is numeric), but the "ID"
    # field is always a string.
    return cast(str, self.rg()["ID"])

to_path

to_path(path: Optional[Path] = None, index: bool = True, pred: Callable[[AlignedSegment], bool] = lambda r: True, tmp_file_type: Optional[SamFileType] = None) -> Path

Write the accumulated records to a file, sorts & indexes it, and returns the Path. If a path is provided, it will be written to, otherwise a temporary file is created and returned.

If path is provided, tmp_file_type may not be provided. In this case, the file type (SAM/BAM/CRAM) will be automatically determined by the file extension when a path is provided. See ~pysam for more details.

If path is not provided, the file type will default to BAM unless tmp_file_type is provided.

Parameters:

Name	Type	Description	Default
path	Optional[Path]	a path at which to write the file, otherwise a temp file is used.	None
index	bool	if True and sort_order is Coordinate and output is a BAM/CRAM file, then an index is generated, otherwise not.	True
pred	Callable[[AlignedSegment], bool]	optional predicate to specify which reads should be output	lambda r: True
tmp_file_type	Optional[SamFileType]	the file type to output when a path is not provided (default is BAM)	None

Returns:

Name	Type	Description
Path	Path	The path to the sorted (and possibly indexed) file.

Source code in fgpyo/sam/builder.py

def to_path(  # noqa: C901
    self,
    path: Optional[Path] = None,
    index: bool = True,
    pred: Callable[[AlignedSegment], bool] = lambda r: True,
    tmp_file_type: Optional[sam.SamFileType] = None,
) -> Path:
    """Write the accumulated records to a file, sorts & indexes it, and returns the Path.
    If a path is provided, it will be written to, otherwise a temporary file is created
    and returned.

    If `path` is provided, `tmp_file_type` may not be provided. In this case, the file type
    (SAM/BAM/CRAM) will be automatically determined by the file extension when a path
    is provided.  See `~pysam` for more details.

    If `path` is not provided, the file type will default to BAM unless `tmp_file_type` is
    provided.

    Args:
        path: a path at which to write the file, otherwise a temp file is used.
        index: if True and `sort_order` is `Coordinate` and output is a BAM/CRAM file, then
               an index is generated, otherwise not.
        pred: optional predicate to specify which reads should be output
        tmp_file_type: the file type to output when a path is not provided (default is BAM)

    Returns:
        Path: The path to the sorted (and possibly indexed) file.
    """
    if path is not None:
        # Get the file type if a path was given (in this case, a file type may not be
        # provided too)
        if tmp_file_type is not None:
            raise ValueError("Both `path` and `tmp_file_type` cannot be provided.")
        tmp_file_type = sam.SamFileType.from_path(path)
    elif tmp_file_type is None:
        # Use the provided file type
        tmp_file_type = sam.SamFileType.BAM

    # Get the extension, and create a path if none was given
    ext = tmp_file_type.extension
    if path is None:
        with NamedTemporaryFile(suffix=ext, delete=False) as fp:
            path = Path(fp.name)

    with NamedTemporaryFile(suffix=ext, delete=True) as fp:
        file_handle: IO
        if self._sort_order in {SamOrder.Unsorted, SamOrder.Unknown}:
            file_handle = path.open("w")
        else:
            file_handle = fp.file

        with sam.writer(file_handle, header=self._samheader, file_type=tmp_file_type) as writer:
            for rec in self._records:
                if pred(rec):
                    writer.write(rec)

        samtools_sort_args = ["-o", str(path), fp.name]

        file_handle.close()
        if self._sort_order == SamOrder.QueryName:
            pysam.sort("-n", *samtools_sort_args)
        elif self._sort_order == SamOrder.Coordinate:
            if index and tmp_file_type.indexable:
                samtools_sort_args.insert(0, "--write-index")
            pysam.sort(*samtools_sort_args)

    return path

to_sorted_list

to_sorted_list() -> List[AlignedSegment]

Returns the accumulated records in coordinate order.

Source code in fgpyo/sam/builder.py

def to_sorted_list(self) -> List[pysam.AlignedSegment]:
    """Returns the accumulated records in coordinate order."""
    with NamedTemporaryFile(suffix=".bam", delete=True) as fp:
        filename = fp.name
        path = self.to_path(path=Path(filename), index=False)
        bam = sam.reader(path)
        return list(bam)

to_unsorted_list

to_unsorted_list() -> List[AlignedSegment]

Returns the accumulated records in the order they were created.

Source code in fgpyo/sam/builder.py

def to_unsorted_list(self) -> List[pysam.AlignedSegment]:
    """Returns the accumulated records in the order they were created."""
    return list(self._records)